Effects of lysine analogs on Penicillium chrysogenum.
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چکیده
منابع مشابه
Effects of lysine analogs on Penicillium chrysogenum.
Compounds structurally related to lysine were tested against Penicillium chrysogenum Wis. 54-1255 for inhibition of growth, sporulation, and penicillin formation. This strain is relatively resistant to lysine analogs. The compounds that were the more active inhibitors of growth and whose activities were reversed by L-lysine were diaminohexynoic acid, N-epsilon-methyllysine, N-alpha-methyllysine...
متن کاملDifferential effects of general amino acid control of lysine biosynthesis on penicillin formation in strains of Penicillium chrysogenum.
The biosynthesis of penicillin by strains Penicillium chrysogenum requires a supply of of the amino acids valine, cysteine and a-aminoadipic acid1}. a-Aminoadipic acid has received most attention since it is an intermediate of lysine biosynthesis in fungi2) and therefore forms a branch point between lysine and penicillin biosynthesis in P. chrysogenumz~6). Higher producing strains of P. chrysog...
متن کاملPenicillin acyltransferase in Penicillium chrysogenum.
Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase was purified threefold by adsorption on calcium phosphate gel at pH 6 and elutio...
متن کاملHomocitrate synthase from Penicillium chrysogenum. Localization, purification of the cytosolic isoenzyme, and sensitivity to lysine.
Subcellular fractionation of cell-free extracts obtained by nitrogen cavitation showed that Penicillium chrysogenum Q176 contains a cytosolic as well as a mitochondrial homocitrate synthase activity. The cytosolic isoenzyme was purified about 500-fold, and its kinetic and molecular properties were investigated. Native homocitrate synthase shows a molecular mass of 155 +/- 10 kDa as determined b...
متن کاملInhibition and repression of homocitrate synthase by lysine in Penicillium chrysogenum.
Homocitrate synthase in the first enzyme of the lysine biosynthetic pathway. It is feedback regulated by L-lysine. Lysine decreases the biosynthesis of penicillin (determined by the incorporation of [14C]valine into penicillin) by inhibiting and repressing homocitrate synthase, thereby depriving the cell of alpha-aminoadipic acid, a precursor of penicillin. Lysine feedback inhibited in vivo the...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1977
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.34.6.706-709.1977